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human colon mucosal epithelial cell line ncm460  (ATCC)


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    ATCC human colon mucosal epithelial cell line ncm460
    Human Colon Mucosal Epithelial Cell Line Ncm460, supplied by ATCC, used in various techniques. Bioz Stars score: 97/100, based on 1432 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/normal+human+colon+mucosal+epithelial+cell+line+ncm460/Primary+Small+Airway+Epithelial+Cells%3B+Normal%2C+Human/pm41148321-48-41-51
    Average 97 stars, based on 1432 article reviews
    human colon mucosal epithelial cell line ncm460 - by Bioz Stars, 2026-09
    97/100 stars

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    Cell Culture:

    Article Title: Long non-coding RNA LINC00511 facilitates colon cancer development through regulating microRNA-625-5p to target WEE1
    Article Snippet: .. Human CC cells SW480, SW620, HCT16, and HT29 and normal human colon mucosal epithelial cell line NCM460 (ATCC, VA, USA) were obtained from ATCC (Manassas, VA, USA) and cultured as per their instructions. ..



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    (A) IC 50 values of HCT-8/V and parental HCT-8 cells for vincristine, taxol, and cisplatin; RI represents the resistance index. (B) Rh123 efflux was measured using flow cytometry. (C) Western blot analysis of ABCB1 and ABCC1; GAPDH was used as a loading control. (D) Increased actin microfilament was observed in the HCT-8/V cells by cytoskeleton staining. (E) Immunofluorescence staining of <t>epithelial</t> and mesenchymal markers in HCT-8 and HCT-8/V cells. Cells were double-stained for vimentin (red) and E-cadherin (green). Nuclei were counterstained blue with DAPI. (F) Expression of EMT markers and transcription factors was determined by Western blot. GAPDH was used as a loading control. Scale bars represent 10 μm in D and E . Data from three independent experiments were graphed as the mean ± SD. * P < 0.05.
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    (A) IC 50 values of HCT-8/V and parental HCT-8 cells for vincristine, taxol, and cisplatin; RI represents the resistance index. (B) Rh123 efflux was measured using flow cytometry. (C) Western blot analysis of ABCB1 and ABCC1; GAPDH was used as a loading control. (D) Increased actin microfilament was observed in the HCT-8/V cells by cytoskeleton staining. (E) Immunofluorescence staining of <t>epithelial</t> and mesenchymal markers in HCT-8 and HCT-8/V cells. Cells were double-stained for vimentin (red) and E-cadherin (green). Nuclei were counterstained blue with DAPI. (F) Expression of EMT markers and transcription factors was determined by Western blot. GAPDH was used as a loading control. Scale bars represent 10 μm in D and E . Data from three independent experiments were graphed as the mean ± SD. * P < 0.05.
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    (A) IC 50 values of HCT-8/V and parental HCT-8 cells for vincristine, taxol, and cisplatin; RI represents the resistance index. (B) Rh123 efflux was measured using flow cytometry. (C) Western blot analysis of ABCB1 and ABCC1; GAPDH was used as a loading control. (D) Increased actin microfilament was observed in the HCT-8/V cells by cytoskeleton staining. (E) Immunofluorescence staining of epithelial and mesenchymal markers in HCT-8 and HCT-8/V cells. Cells were double-stained for vimentin (red) and E-cadherin (green). Nuclei were counterstained blue with DAPI. (F) Expression of EMT markers and transcription factors was determined by Western blot. GAPDH was used as a loading control. Scale bars represent 10 μm in D and E . Data from three independent experiments were graphed as the mean ± SD. * P < 0.05.

    Journal: Oncotarget

    Article Title: Twist1 confers multidrug resistance in colon cancer through upregulation of ATP-binding cassette transporters

    doi: 10.18632/oncotarget.17548

    Figure Lengend Snippet: (A) IC 50 values of HCT-8/V and parental HCT-8 cells for vincristine, taxol, and cisplatin; RI represents the resistance index. (B) Rh123 efflux was measured using flow cytometry. (C) Western blot analysis of ABCB1 and ABCC1; GAPDH was used as a loading control. (D) Increased actin microfilament was observed in the HCT-8/V cells by cytoskeleton staining. (E) Immunofluorescence staining of epithelial and mesenchymal markers in HCT-8 and HCT-8/V cells. Cells were double-stained for vimentin (red) and E-cadherin (green). Nuclei were counterstained blue with DAPI. (F) Expression of EMT markers and transcription factors was determined by Western blot. GAPDH was used as a loading control. Scale bars represent 10 μm in D and E . Data from three independent experiments were graphed as the mean ± SD. * P < 0.05.

    Article Snippet: The normal human colon mucosal epithelial cell line (NCM460), the low invasive human colon adenocarcinoma cell line (HT-29), the highly invasive human colon cancer cells (HCT-8), HCT-8/V and Bel7402 hepatocarcinoma cell line were purchased from KeyGEN BioTECH (Nanjing, China).

    Techniques: Flow Cytometry, Western Blot, Control, Staining, Immunofluorescence, Expressing